To a certain extent it is possible, if say u had 2 other pc's, the pc with the 2 nic's in it can actually act like a bridge between the 2 other pc's, allowing you to download from one pc and upload to the other simultaneously, without dropping the transfer rate, as you are using 2 separate cards to do the file transfers. This eliminates the need for a hub on a 3 pc network. However, I don't think its possible on a 2 pc network to configure 1 nic to upload and another to download, I may be wrong however, but i've never heard of any such configuration