Yes, but I wouldn't recommend it. I had to use both Alky for Applications and OS Spoofer to get it to run. In case you don’t know what Alky for Applications is, it basically is a plugin that allowed Windows XP to run Vista applications like the Windows Sidebar that would allow you to run Vista gadgets, but it somewhat runs on Windows 2000 w/KernelEx, albeit poorly (not in this case, though). First, spoof the win2k installation to xp, then if you haven't installed Alky already (Link to download Alky), make sure to install it first before installing Spotify. Then, install Spotify. You can then spoof it back to win2k if you want. Don't just directly open Spotify up, Right click on the desktop shortcut and select "Run Vista Executable..." in the menu. It runs, but its a bit buggy. Note that I tested it in VMWare, how sure how it would run on real hardware.