I have read documents high and low, searched up and down ... yet not found anything that can help me with my issue. I keep getting this error message, which doesn't seem to be very uncommon. HTTP Error 401.3 - Unauthorized: Access is denied due to an ACL set on the requested resource. Now, I have fixed the permissions for ASP.Net 2.0, the only remaining piece is those in the c:\inetpub\wwwroot\. Say I copy my web projects to a subfolder in wwwroot, try to access it and I get the error, but when i mark a file, individually add permission for the "User" account, I can then access the file locally and from outside the network. This is rather tedious, should this not be done automatically? How can I fix this? It's a brand new & clean installation of IIS 6.0.