No, I mean a real DOS envirenment. I boot from a 64 Mega Byte (yes, it's that small) USB thumbdrive into MSDOS (Win 98 version).
I believe my boot image is probably actually from an old 600MB HDD I once ran with on a AMD 486DX CPU. It shows up as a hard drive instead of a floppy.
I do have several floppy boot images as well (*.img), but I do remember copying the MBR from a HDD and putting it on a thumbdrive once upon a time.
Also I've found running Ghost and other programs work better on thumbdrive, because some programs want to save stuff to the boot drive, and CD-ROM would cause an error (I forget which exact program I used that did that).
I just used Ghost32.exe on my Windows 10 64-Bit PC to Ghost my Laptop drive partitions the other day. I just ran it as a normal program inside Windows 10 GUI, no Dosbox or anything. Lucky for me it is a small laptop HDD (250GB), so it works fine. Unfortunately another drive I have has Win10 and it is a 2TB Drive. The partition is less than 1TB, but Ghost says it's -something, a negative number. When I tried backing that partition up, it just saved a 6kb sized file with nothing but the drive location, drive name, and the partition was named "unknown", no files.
My understanding from reading the previous posts is that I would need to run Ghost2003 on a Windows 98 desktop to use VFAT.Vxd to be able to backup the partitions on a 2TB drive.